期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 5, 期 6, 页码 768-777出版社
WILEY
DOI: 10.1111/j.1467-7652.2007.00283.x
关键词
low-calorie sweetener; miracle fruit; miraculin; sweetness-inducing activity; taste-modifying protein; transgenic tomato
Miraculin is a taste-modifying protein isolated from the red berries of Richadella dulcifica, a shrub native to West Africa. Miraculin by itself is not sweet, but it is able to turn a sour taste into a sweet taste. This unique property has led to increasing interest in this protein. In this article, we report the high-yield production of miraculin in transgenic tomato plants. High and genetically stable expression of miraculin was confirmed by Western blot analysis and enzyme-linked immunosorbent assay. Recombinant miraculin accumulated to high levels in leaves and fruits, up to 102.5 and 90.7 mu g/g fresh weight, respectively. Purified recombinant miraculin expressed in transgenic tomato plants showed strong sweetness-inducing activity, similar to that of native miraculin. These results demonstrate that recombinant miraculin was correctly processed in transgenic tomato plants, and that this production system could be a good alternative to production from the native plant.
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