期刊
PROTEOMICS
卷 7, 期 22, 页码 4066-4068出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200700484
关键词
ovarian cancer; protein extraction buffer; reverse phase protein microarray
资金
- Intramural NIH HHS Funding Source: Medline
Application of novel technology to clinical samples requires optimization of procedures. Reverse phase protein lysate arrays use femtomolar quantities of tissue lysate from clinical samples with which to profile biochemical events happening in the tumor. We analyzed the effects of different tissue solubilization buffers on frozen ovarian tumor samples in order to identify the system with the best signal intensity dynamic range, reproducibility, tissue solubility, and signal consistency. A modified RIPA-like buffer supplemented with DTT and SDS was deemed optimal.
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