期刊
REPRODUCTION
卷 134, 期 5, 页码 695-704出版社
BIOSCIENTIFICA LTD
DOI: 10.1530/REP-07-0259
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资金
- PHS HHS [R03] Funding Source: Medline
During fertilization of mammalian eggs a factor from the sperm, the sperm factor (SF), is released into the ooplasm and induces persistent [Ca2+](i) oscillations that are required for egg activation and embryo development. A sperm-specific phospholipase C (PLC), PLC zeta, is thought to be the SF. Here, we investigated whether the SF activity and PLC zeta are simultaneously and completely released into the ooplasm soon after sperm entry. To accomplish this, we enucleated sperm heads within 90 min of intracytoplasmic sperm injection (ICSI) and monitored the persistence of the [Ca2+](i) oscillations in eggs in which the sperm had been withdrawn. We also stained the enucleated sperm heads to ascertain the presence/absence of PLC zeta. Our results show that by 90 min all the SF activity had been released from the sperm, as fertilized enucleated eggs oscillated as fertilized controls, even in cases in which oscillations were prolonged by arresting eggs at metaphase. In addition, we found that the released SF activity became associated with the pronucleus (PN), as induction of PN envelope breakdown evoked comparable [Ca2+](i) responses in enucleated and non-manipulated zygotes. Lastly, we found that PLC zeta localized to the equatorial area of bull sperm and to the post-acrosomal region of mouse sperm and that by 90 min after ICSI all the sperm's PLC zeta immunoreactivity was lost in both species. Altogether, our findings show that during fertilization the SF activity and PLC zeta immunoreactivity are simultaneously released from the sperm, suggesting that PLC zeta may be the only [Ca2+](i) oscillation-inducing factor of mammalian sperm.
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