期刊
CARBOHYDRATE RESEARCH
卷 342, 期 15, 页码 2322-2325出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2007.06.020
关键词
beta-elimination; ammonia; O-glycopeptide; MALDI-TOF MS; IgA
Pools of O-glycopeptides (and their deglycosylated analogues) derived from trypsin-digested normal human serum IgA1 have been treated with ammonia under conditions reported to result in complete liberation of O-glycans linked to serine and threonine residues in glycopeptides and glycoproteins. MALDI-TOF MS analysis has revealed that only one of the six glycosylated sites is susceptible to beta-elimination under these conditions. It is likely that resistance to beta-elimination is due to very close proximity of proline to the glycosylated serine or threonine residues. Preliminary results using 0.1 M NaOH (instead of ammonia) to perform beta-elimination indicated that there was also selective de-O-glycosylation with this reagent, however, these results were complicated by the concomitant hydrolysis of the peptide bonds. These findings may have implications for similarly O-glycosylated peptides and proteins and possibly for other chemical methods that are used to carry out beta-eliminations of O-glycans. (C) 2007 Elsevier Ltd. All rights reserved.
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