期刊
CHEMBIOCHEM
卷 8, 期 16, 页码 1997-2002出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200700402
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This paper describes a straightforward procedure to immobilize oligonucleotides on glass substrates in well-defined micropatterns by microcontact printing with a dendrimer-modified stomp. The oligonucleotides are efficiently immobilized by click chemistry induced by microcontact printing. Acetylene-modified oligonucleotides were treated with an azide-terminated gloss slide under the confinement of the dendrimer-modified stomp, without the use of a Cu-I catalyst. The immobilization is on irreversible, covalent, and one-step reaction that results in stable attachment of: the oligonucleotides. Oligonucleotides with the acetylene-modification at the 5' terminus hybridize selectively with full-length, complementary targets. Strands with more than one acetylene linker do not hybridize with complementary strands.
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