期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 17, 期 22, 页码 6147-6150出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2007.09.035
关键词
peptide; protein; ITC; calorimetry; multivalent; bivalent; PDZ
资金
- NIGMS NIH HHS [R01 GM063021-01A1, R01 GM063021, R01 GM063021-02, GM 63021, R01 GM063021-06, R01 GM063021-05, R01 GM063021-03, R01 GM063021-04] Funding Source: Medline
A series of multivalent peptides, with the ability to simultaneously bind two separate PDZ domain proteins, has been designed, synthesized, and tested by isothermal titration calorimetry (ITC). The monomer sequences, linked with succinate, varied in length from five to nine residues. The thermodynamic binding parameters, in conjunction with results from mass spectrometry, indicate that a ternary complex is formed in which each peptide arm binds two equivalents of the third PDZ domain (PDZ3) of the neuronal protein PSD-95. (C) 2007 Elsevier Ltd. All rights reserved.
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