4.5 Article

Depletion of topoisomerase IIα leads to shortening of the metaphase interkinetochore distance and abnormal persistence of PICH-coated anaphase threads

期刊

JOURNAL OF CELL SCIENCE
卷 120, 期 22, 页码 3952-3964

出版社

COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.013730

关键词

topoisomerase; PICH; chromosome; centromere; condensation; segregation

资金

  1. Biotechnology and Biological Sciences Research Council [BBS/B/04994] Funding Source: Medline
  2. Cancer Research UK [A5962] Funding Source: Medline
  3. Biotechnology and Biological Sciences Research Council [BBS/B/04994] Funding Source: researchfish

向作者/读者索取更多资源

Topoisomerase II (topo II) is a major component of mitotic chromosomes, and its unique decatenating activity has been implicated in many aspects of chromosome dynamics, of which chromosome segregation is the most seriously affected by loss of topo II activity in living cells. There is considerable evidence that topo II plays a role at the centromere including: the centromere-specific accumulation of topo II protein; cytogenetic/molecular mapping of the catalytic activity of topo II to active centromeres; the influence of sumoylated topo II on sister centromere cohesion; and its involvement in the activation of a Mad2-dependent spindle checkpoint. By using a human cell line with a conditional-lethal mutation in the gene encoding DNA topoisomerase II alpha, we find that depletion of topo II alpha, while leading to a disorganised metaphase plate, does not have any overt effect on general assembly of kinetochores. Fluorescence in situ hybridisation suggested that centromeres segregate normally, most segregation errors being chromatin bridges involving longer chromosome arms. Strikingly, a linear human X centromere-based minichromosome also displayed a significantly increased rate of missegregation. This sensitivity to depletion of topo II alpha might be linked to structural alterations within the centromere domain, as indicated by a significant shortening of the distance across metaphase sister centromeres and the abnormal persistence of PICH-coated connections between segregating chromatids.

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