We measure the forces confining the displacement of a single DNA molecule embedded within a concentrated solution of long relaxed circular DNA molecules (115 kbp at 1 mg/mL) using optical tweezers. We compare these measurements with our previous measurements of forces imposed by entangled linear DNA molecules of the same length and concentration. A tube-like confining field and three relaxation modes were observed, but the tube radius was 25% smaller (congruent to 0.6 mu m) and the longest relaxation time similar to 3 times shorter (congruent to 11 s) than with linear molecules, consistent with recent theoretical predictions by Iyer, Lele, and Juvekar. For displacements greater than the tube radius, the confining force imposed by circular polymers was substantially lower and shorter range than that measured with linear polymers.
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