期刊
FEBS LETTERS
卷 581, 期 28, 页码 5469-5474出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.10.052
关键词
FT-IR imaging; histopathology; cancer; metabolism; CAM-assay; oxidative stress
Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio-allantoic membrane. In cell culture, glucose consumption and lactic acid release were higher in U87 cells. A549/8 cells were less sensitive to oxidative stress as observed through changes in fatty acyl chains. In vivo biochemical mapping of highly ( U87) vs. poorly (A549/8) angiogenic tumors provided results comparable to culture models. Therefore, FT-IR imaging allows detecting subtle chemical changes in tumors, which might be useful for diagnosis. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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