期刊
JOURNAL OF NEUROSCIENCE
卷 27, 期 48, 页码 13098-13107出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3986-07.2007
关键词
dendrite; Wnt; Par1b/MARK2; dishevelled; translocation; MAP2
Neurons are highly polarized cells that possess two morphologically and functionally different types of protrusions, axons and dendrites, that function in the transmission and reception of neural signals, respectively. A great deal of attention has been paid to the specification and guidance of axons, but the mechanism of dendrite development remains mostly unknown. We report here that a polarity- regulating kinase, partitioning- defective 1 (Par1b)/ microtubule affinity- regulating kinase 2 ( MARK2), specifically regulates development of dendrites in hippocampal neurons. Ectopic expression of Par1b/ MARK2 shortens the length and decreases branching of dendrites without significant effects on axons. Knockdown of endogenous Par1b/ MARK2 by RNA interference stimulates dendrite development. Wnt stimulation and Dishevelled expression, both of which are known to induce dendrite development, induced recruitment of Par1b/ MARK2 to the membrane fraction. Expression of a Par1b/ MARK2 mutant, that contains a myristoylation signal and accumulates exclusively in membranes, does not affect dendrite development. In addition, Par1b/ MARK2 efficiently phosphorylated MAP2, which is localized mainly in dendrites. These results indicate that Par1b/ MARK2 negatively regulates dendrite development through phosphorylation of MAP2.
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