期刊
MOLECULAR CELL
卷 28, 期 4, 页码 692-699出版社
CELL PRESS
DOI: 10.1016/j.molcel.2007.10.009
关键词
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资金
- NIGMS NIH HHS [R01 GM058921, 5F32GM070101, GM27789, GM067761-01S1, GM067761] Funding Source: Medline
Metazoan replication-dependent histone mRNAs; are not polyadenylated and instead end in a conserved stem loop that is the cis element responsible for coordinate posttranscriptional regulation of these mRNAs. Using biochemical approaches, only a limited number of factors required for cleavage of histone pre-mRNA have been identified. We therefore performed a genome-wide RNA interference screen in Drosophila cells using a GFP reporter that is expressed only when histone pre-mRNA processing is disrupted. Four of the 24 genes identified encode proteins also necessary for cleavage/polyadenylation, indicating mechanistic conservation in formation of different mRNA3 ' ends. We also unexpectedly identified the histone variants H2Av and H3.3A/B. In H2Av mutant cells, U7 snRNP remains active but fails to accumulate at the histone locus, suggesting there is a regulatory pathway that coordinates the production of variant and canonical histones that acts via localization of essential histone pre-mRNA processing factors.
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