期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 363, 期 4, 页码 1027-1032出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2007.09.077
关键词
tat; HA2; protein transduction domain; PTD; shepherdin; peptidomimetic antagonist; peptide blocker
Tat peptides are useful carriers for delivering biologic molecules into the cell for both functional analysis of intracellular disease-related proteins and treatment of refractory diseases. Most internalized Tat-fused cargos (Tat-cargos) are trapped within the endosome, however, which limits the biologic function of the cargo. In this study, we demonstrated that Tat-fused HA2 peptide (HA2(Tat)), an endosome disrupted peptide, enhanced the endosome-escape efficiency of Tat-cargos. In cells treated with a mixture of fluorescein isothiocyanate-labeled Tat and HA2 Tat, widespread fluorescence was observed throughout the cytosol. In addition, this HA2(Tat)-mediated cytosolic delivery technique led to enhanced cytotoxicity of Tat-fused anti-cancer peptides, specifically shepherdin. Thus, we improved the function of the delivered molecules by co-treating with HA2(Tat) and propose that this is a useful method for the delivery of therapeutic macromolecules into the cytosol. (C) 2007 Elsevier Inc. All rights reserved.
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