期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 89, 期 3, 页码 570-577出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.13-0239
关键词
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资金
- Fundacao de Amparo a Pesquisa de Minas Gerais (FAPEMIG), Belo Horizonte, Minas Gerais, Brazil [APQ-01562-11]
- FAPEMIG
- PDTIS-FIOCRUZ (Rede de Plataformas Tecnologicas do Programa de Desenvolvimento Tecnologico em Insumos para Saude) [RID06]
- National Counsel of Technological and Scientific Development (CNPq) [311641/2009-1]
The aim of this study was to evaluate the accuracy of invasive and non-invasive tests for diagnosis of visceral leishmaniasis (VL) in a large series of human immunodeficiency virus (HIV)-infected patients. In this,delayed-type cross-sectional study, 113 HIV-infeeted symptomatic patients were evaluated by an adjudication committee after clinical follow-up to establish the presence or absence of VL as the target condition (reference test). The index tests were recombinant K39 antigen-based immunochromatographic test (rK39), indirect fluorescent antibody test (IFAT), prototype kit of direct agglutination test (DAT-LPC), and real-time polymerase chain reaction (qPCR) in peripheral blood. Compared with parasitological test and adjudication committee diagnosis or latent class model analyses, IFAT and rk39 dipstick test presented the lowest sensitivity. DAT-LPC exhibited good overall performance, and there was no Statistical difference between DAT-LPC and qPCR diagnosis accuracy. Real-time PCR emerges as a less invasive alternative to parasitological examination for confirmation of cases not identified by DAT.
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