期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 86, 期 1, 页码 140-151出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2012.11-0503
关键词
-
资金
- National Institutes of Health [AI065717]
- Fogarty International Center [TW007872, TW007377, TW007735]
- Global Fund to Fight Aids, Tuberculosis and Malaria
- FOGARTY INTERNATIONAL CENTER [D43TW007377, R01TW007872, R25TW007735] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U19AI065717, U19AI089686] Funding Source: NIH RePORTER
Anopheles punctulatus sibling species (An. punctulatus s.s., Anopheles koliensis, and Anopheles farauti species complex [eight cryptic species]) are principal vectors of malaria and filariasis in the Southwest Pacific. Given significant effort to reduce malaria and filariasis transmission through insecticide-treated net distribution in the region, effective strategies to monitor evolution of insecticide resistance among An. punctulatus sibling species is essential. Mutations in the voltage-gated sodium channel (VGSC) gene have been associated with knock-down resistance (kdr) to pyrethroids and DDT in malarious regions. By examining VGSC sequence polymorphism we developed a multiplex assay to differentiate wild-type versus kdr alleles and query intron-based polymorphisms that enable simultaneous species identification. A survey including mosquitoes from seven Papua New Guinea Provinces detected no kdr alleles in any An. punctulatus species. Absence of VGSC sequence introgression between species and evidence of geographic separation within species suggests that kdr must be monitored in each An. punctulatus species independently.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据