期刊
BIOCHEMICAL JOURNAL
卷 408, 期 -, 页码 287-295出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20070555
关键词
assembly; endosome; membrane; recruitment; retromer; sorting
资金
- Medical Research Council [G117/452] Funding Source: researchfish
- MRC [G117/452] Funding Source: UKRI
- Medical Research Council [G117/452] Funding Source: Medline
The retromer complex is a conserved cytoplasmic coat complex that mediates the endosome-to-Golgi retrieval of vacuole/lysosome hydrolase receptors in yeast and mammals. The recognition of cargo proteins by the retromer is performed by the Vps35p/ VPS35 (where Vps is vacuolar protein sorting) component, which together with Vps26p/VPS26 and Vps29p/VPS29, forms the cargo-selective subcomplex. In this report, we have identified a highly-conserved region of Vps35p/VPS35 that is essential for the interaction with Vps26p/VPS26 and for assembly of the retromer complex. Mutation of residues within the conserved region results in Vps35p/VPS35 mutants, which cannot bind to Vps26p/VPS26 and are not efficiently targeted to the endosomal membrane. These data implicate Vps26p/VPS26 in regulating Vps35p/VPS35 membrane association and therefore suggest a role for Vps26p/VPS26 in cargo recognition.
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