期刊
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
卷 39, 期 -, 页码 68-75出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijid.2015.08.012
关键词
Trypanosoma cruzi; Discrete typing units; Chronic chagasic patients; Clinical forms; Genetic diversity
资金
- National Council for Scientific and Technological Development (MCT/CNPq) [472251/2010-4]
- MCTI/CNPq/MS-SCTIE-Decit [404056/2012-1]
- Coordination for the Improvement of Higher Education Personnel (CAPES) - National Incentive Program for Basic Parasitology (Programa Nacional de Incentivo a Parasitologia Basica [23038.005288/2011-48]
- CNPq
- CAPES
- Serido Ecological Station
- Chico Mendes Institute for Biodiversity Conservation (Instituto Chico Mendes de Conservacao da Biodiversidade, ICMBio)
- State Secretary for Public Health of Rio Grande do Norte, Brazil
Objective: Trypanosoma cruzi is subdivided into six discrete typing units (DTUs), TcI-TcVI. The precise identification of each can contribute to tracking wild DTUs that invade the domiciliary environment. Methods: Twenty T. cruzi stocks isolated from 16 chagasic patients, two Panstrongylus lutzi, one Galea spixii, and one Euphractus sexcinctus, from different localities in the State of Rio Grande do Norte, Brazil, were characterized by genotyping the 3' region of the 24S alpha rRNA gene, the mitochondrial cytochrome oxidase subunit 2 gene, and the spliced leader intergenic region. Results: TcIII was identified in 18.7% (3/16) of patients from different municipalities, as well as in P. lutzi, G. spixii, and E. sexcinctus, indicating the connection between the sylvatic and domestic cycles in this Brazilian semi-arid region. TcI and TcII were also detected, in 37.5% (6/16) and 43.8% (7/16) of patients, respectively. These DTUs were associated with cardiac, digestive, and indeterminate clinical forms, while TcIII was identified only in patients with the indeterminate form. Conclusions: The occurrence of these DTUs reveals important phylogenetic diversity in T. cruzi isolates from humans. TcIII is reported for the first time in northeastern Brazil. These findings appear to indicate an overlap between the sylvatic and domestic transmission cycles of the parasite in this region. (C) 2015 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
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