Polyunsaturated fatty acids induce α-synuclein-related pathogenic changes in neuronal cells

The misfolding and aggregation of normally soluble proteins has emerged as a key feature of several neurodegenerative diseases. in Parkinson's disease, progressive loss of dopaminergic neurons is accompanied by polymerization of the cytoplasmic protein a-synuclein (alpha S) into filamentous inclusions found in neuronal somata (Lewy bodies) and dendrites (Lewy neurites). Similar aS aggregates occur in cortical neurons in dementia with Lewy bodies. Numerous reports now indicate that aS can interact with lipids. We previously found that treating dopaminergic cells expressing alpha S with polyunsaturated fatty acids (PUFAs) induced the formation of soluble, sodium dodecyl sulfate-stable oligomers whereas treatment with saturated fatty acids did not Here, we examine the relevance of alpha S-PUFA interactions to the development of Parkinson's disease-like cytopathology. Exposure of alpha S-overexpressing dopaminergic or neuronal cell lines to physiological levels of a PUFA induced the formation of proteinaceous inclusions in the cytoplasm. Kinetic experiments indicated that PUFA-induced soluble oligomers of alpha S precede these Lewy-like inclusions. Importantly, we found that alpha S oligomers were associated with cytotoxicity, whereas the development of Lewy-like inclusions appeared to be protective. We conclude that alterations in PUFA levels can lead to aggregation of alpha S and subsequent deposition into potentially cytotoxic oligomers that precede inclusions in dopaminergic cells.