Studies on the Salvador I Strain of Plasmodium vivax in Non-human Primates and Anopheline Mosquitoes

A review is presented on studies conducted in New World monkeys and chimpanzees with the Salvador I strain of Plasmodium vivax. This isolate has been adapted to Aotus and Saimiri (squirrel) monkeys and developed as a model for the testing of antimalarial vaccines. After the injection of 10,000 sporozoites, the median prepatent period in S. boliviensis monkeys was 21.5 days. In 103 sporozoite-induced infections in splenectomized monkeys, the median maximum parasite count ranged from 2,139 to 202,368/mu L, with a median maximum parasite count of 48,174/mu L. Median maximum parasite counts in Aotus lemitrinus griseimembra, A. nancymaae, A. azarae boliviensis, and A. vociferans monkeys were 19,902, 1873907 21,420, and 18,210/mu L, respectively and ranged from 124 to 156,000/mu L. Mosquito infections were readily obtained in different species of Anopheles mosquitoes. The S boliviensis monkey and Salvador I strain seems suitable for the testing of sporozoite and liver stage vaccines but not for blood-stage vaccines against P vivax unless adapted further in spleen-intact Saimiri boliviensis monkeys.