期刊
HUMAN PATHOLOGY
卷 38, 期 12, 页码 1760-1763出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.humpath.2007.04.004
关键词
JAK2; spleen; extramedullary hematopoiesis; chronic myeloproliferative disorders; chronic myelomonocytic leukemia
类别
Extramedullary hematopoiesis occurs in patients with a variety of hematologic diseases, and the spleen is a common site. Extramedullary hematopoiesis is very common in chronic myeloproliferative diseases and myeloproliferative/myelodysplastic diseases. The pathogenesis of extramedullary hematopoiesis is unknown. Using JAK2 V617F mutation as a molecular marker, we assessed paired spleen and bone marrow samples of 15 patients with various types of chronic myeloproliferative diseases and myeloproliferative/myelodysplastic diseases. The diagnosis was chronic idiopathic myelofibrosis (n = 8), polycythemia vera (n = 3), and chronic myelomonocytic leukemia to = 4). DNA was extracted from fixed, paraffin-embedded tissue and assessed for JAK2 V617F by real-time polymerase chain reaction assay followed by melting curve analysis. Concordant JAK2 mutation was detected in the paired samples in 7 patients. A discordant result with JAK2 V617F found in the spleen but not bone marrow was noted in I patient. These results indicate that extramedullary hematopoiesis in patients with chronic myeloproliferative diseases and myeloproliferative/myelodysplastic diseases is a clonal process and lend support to the theory that the cells of extramedullary hematopoiesis are carried from the bone marrow. (c) 2007 Elsevier Inc. All rights reserved.
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