Lapillicoccus jejuensis gen. nov., sp nov., a novel actinobacterium of the family Intrasporangiaceae, isolated from stone

A novel, yellow-pigmented actinobacterium was isolated from a small stone collected in Jeju, Republic of Korea. The cells of the organism, designated strain R-Ac013(T), were Gram-positive, aerobic, non-motile cocci that occurred singly or in pairs. The strain showed growth at 10-37 degrees C and pH 4.1-11.1, and tolerated 2% NaCl. On the basis of the 16S rRNA gene sequence, the organism was phylogenetically related to members of the genera Tetrasphaera (94.6-97.1% sequence similarity), Terrabacter (96.5%), Knoellia (96.4%), Terracoccus (96.4%), Oryzihumus (96.4%), Janibacter (96.1-96.4%) and Intrasporangium (96.2%). The chemotaxonomic results for the organism were as follows: LL-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan, acetyl-type murein, MK-8(H-4) as the major menaquinone, a DNA G + C content of 74.1 mol%, and a polar lipid profile that comprised diphosphatidylglycerol and phosphatidylinositol. The fatty acid profile consisted of iso- and anteiso-methyl-branched, straight-chain saturated and monounsaturated types, the major components being iso-C-16:0, C-17:1 omega 8c and iso-C-15:0. The combination of the phenotypic and phylogenetic data revealed that this strain represents a novel genus and species of the family Intrasporangiaceae, for which the name Lapillicoccus jejuensis gen. nov., sp. nov. is proposed. The type strain is strain R-Ac013(T) (=KCTC 19200(T) =DSM 18607(T)).