Rapid prototyping of microfluidic systems using a laser-patterned tape

We introduce a laser-patterned tape as a master for replica moulding microfluidics in poly( dimethylsiloxane) ( PDMS). Normally, a laser is applied to scribe microchannels directly on poly( methyl methacrylate) ( PMMA) substrates. This direct engraving usually offers a faster turn-around time than conventional soft lithography but generates rough surfaces which perform poorly under phase-contrast microscopy imaging. Using a laser-patterned tape as the master template for replica-moulding microfluidics in PDMS, we combine the rapid turn-around time of laser ablation and relatively smooth surface finish of soft lithography. Hence, microfluidic devices suitable for optical microscopy imaging can be obtained within several hours.