期刊
FUNGAL GENETICS AND BIOLOGY
卷 44, 期 12, 页码 1355-1367出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2007.07.012
关键词
Pac1 mutants; pH signalling; acidification; ammonium utilization; phytopathogenic fungal antagonism
In Trichoderma harzianum CECT 2413 external pH regulates essential functions such as growth rate, sporulation, cell and colony morphology, pattern of secreted proteins, gene expression or mycoparasitism-related enzymatic activities. pH regulation is mediated by the transcriptional factor Pac1 (homologous to PacC regulator in other fungi), encoded by pacl whose expression increases with pH. Two pac1 mutants have been obtained from CECT 2413: P2.32, that possesses an allele of pac1 active at any pH, and R 13, that does not express pacl due to interferent RNA. Cell morphology, sporulation and growth rate are optimal for strain P2.32 at pH 7.5 and for strain R13 at pH 3.0, mimicking alkaline and acidic conditions, respectively. Pact regulates some genes involved in antagonism: chit42 chitinase, papA protease, gtt1 glucose permease, and qid74 cell wall protein. Further-more, Pacl modulates T. harzianum antifungal activity since wild type and mutants inhibit several phytopathogenic fungal strains at various degrees in different assays. (c) 2007 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据