期刊
NEW ZEALAND JOURNAL OF MARINE AND FRESHWATER RESEARCH
卷 41, 期 4, 页码 385-390出版社
RSNZ PUBLISHING
DOI: 10.1080/00288330709509928
关键词
fluorescent in situ hybridisation (FISH); sandwich hybridisation assay (SHA); harmful algal blooms; oligonucleotide probes
The dinoflagellate Gymnodinium catenatum was first observed in New Zealand at Manukau Harbour on the west coast of the North Island in May 2000. At that time, a strong correlation was evident between the micro-algal bloom and the occurrence of paralytic shellfish toxins (PSP) in shellfish. This paper describes the design and testing of oligonucletide probes targeting the large sub-unit (LSU) ribosomal RNA (rRNA) of G. catenatum. The probes were developed in fluorescent in situ hybridisation (FISH) and sandwich hybridisation assay (SHA) format to rapidly differentiate the target PSP producer from non-toxic look-alike dinoflagellates. Specificity was affirmed by testing the probes against dinoflagellate and flagellate isolates.
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