期刊
JOURNAL OF VIROLOGY
卷 81, 期 23, 页码 13271-13276出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01647-07
关键词
-
类别
Crimean-Congo hemorrhagic fever virus (CCHFV) causes severe human disease. The CCHFV medium RNA encodes a polyprotein which is proteolytically processed to yield the glycoprotein precursors PreGn and PreGc, followed by structural glycoproteins Gn and Gc. Subtilisin kexin isozyme-1/site-1 protease (SKI-1/SIP) plays a central role in Gn processing. Here we show that CCHFV-infected cells deficient in SKI-1/SIP produce no infectious virus, although PreGn and PreGc accumulated normally in the Golgi apparatus, the site of virus assembly. Only nucleoprotein-containing particles which lacked virus glycoproteins (Gn/Ge or PreGn/PreGc) were secreted. Complementation of SKI-1/SlP-deficient cells with a SKI-1/SIP expression vector restored release of infectious virus (> 10(6) PFU/ml), confirming that SKI-1/SIP processing is required for incorporation of viral glycoproteins. SKI-1/SIP may represent a promising antiviral target.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据