期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 143, 期 3, 页码 212-223出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-007-8003-4
关键词
directed evolution; DNA shuffling; in vitro recombination; mutagenesis; random priming
The engineering of enzymes with altered activity, specificity, and stability, using directed evolution techniques that mimic evolution on a laboratory timescale, is now well established. In vitro recombination techniques such as DNA shuffling, staggered extension process (StEP), random chimeragenesis on transient templates (RACHITT), iterative truncation for the creation of hybrid enzymes (ITCHY), recombined extension on truncated templates (RETT), and so on have been developed to mimic and accelerate nature's recombination strategy. This review discusses gradual advances in the techniques and strategies used for the directed evolution of biocatalytic enzymes aimed at improving the quality and potential of enzyme libraries, their advantages, and disadvantages.
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