期刊
NANO LETTERS
卷 7, 期 12, 页码 3875-3878出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl071943y
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资金
- BBSRC [BB/D010918/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D010918/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/D001846/1] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/D010918/1] Funding Source: Medline
We form artificial lipid bilayers suitable for single-molecule fluorescence microscopy by contacting an aqueous droplet with a hydrogel support immersed in a solution of lipid in oil. Our results show that droplet on hydrogel bilayers (DHBs) have high lipid mobilities, similar to those observed in unsupported lipid bilayers. DHBs are also stable over a period of several weeks. We examine membrane protein diffusion in these bilayers and report a decreased lateral mobility of the heptameric beta-barrel pore-forming toxin (x-hemolysin versus that of its monomeric precursor. These results corroborate previous models of the a-hemolysin insertion mechanism where the monomer binds to the lipid bilayer without insertion.
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