期刊
CHEMISTRY AND PHYSICS OF LIPIDS
卷 150, 期 2, 页码 143-155出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2007.06.224
关键词
algae; lipids; MALDI-TOF MS; TLC
The lipid composition of algae is crucial for numerous structural and physiological aspects, e.g. the integrity of the photosynthetic complexes and the functionality of membrane-embedded processes as the photosynthetic electron transport in thylakoids or the mitochondrial respiration. In this paper the lipid composition of the organic extracts of the green alga Chlamydomonas reinhardtii and the diatom Cyclotella meneghiniana are compared by using matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) in combination with thin-layer chromatography (TLC). The combined methods enable quantitative evaluation of the individual lipid classes as well as the determination of the relative acyl compositions. It will be shown that both algae differ in (a) the lipid classes, (b) the relative contribution of the individual lipid classes and (c) the acyl compositions. Differences in the acyl composition concern particularly the mono- and digalactosyl diacylglycerols. Glycerol-trimethylhomoserine and phosphatidylethanolamine are exclusively detected in the C. reinhardtii extracts, whereas phosphatidylcholine is a characteristic lipid of C. meneghiniana. Furthermore, the proportion of the acidic lipids sulfoquinovosyl-diacylglycerol and phosphatidylglycerol is significantly higher in the diatom than in C. reinhardtii. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
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