期刊
TOXICOLOGY LETTERS
卷 175, 期 1-3, 页码 24-33出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2007.09.008
关键词
ultrafine amorphous silica particles; lung inflammation; cytokines; chemokines
类别
资金
- Food & Drug Administration (KFDA), Republic of Korea [07141나노독761] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In order to evaluate the pulmonary effects and inflammatory mechanisms of ultrafine amorphous silica particles (UFASs), the UFASs suspension was prepared in PBS and intratracheally administered to AM mice at doses of 0, 2, 10 and 50 mg/kg (n = 5 per group). Animals were sacrificed at 24 h, and 1, 4 or 14 weeks following exposures. At each time point, a bronchoalveolar lavage fluid analysis, histopathological examination, quantitative real-time PCR and immunohistochemistry of the lung tissues were assessed. The intratracheal instillation of UFASs significantly increased the lung weights and total BAL cells following exposures. The histopathological examination revealed that UFASs-induced severe inflammation, with neutrophils, at an early stage and chronic granulomatous inflammation at the later stage. The mRNA and protein levels of IL-1 beta, IL-6, IL-8, TNF-alpha, MCP-1 and MIP-2 in lung tissues were significantly increased during the early stages, but there were no changes after weeks 1 (TNF-alpha) or 4 (IL-1 beta, IL-6, IL-8, MCP-1 and MIP-2). Instillation of UFASs-induced transient, but very severe lung inflammation. Therefore, the cytokines (IL-1 beta, IL-6. IL-8 and TNF-alpha) and chemokines (MCP-1 and MIP-2) play important roles in the inflammation induced by the intratracheal instillation of UFASs. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
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