期刊
BRAIN RESEARCH
卷 1185, 期 -, 页码 33-44出版社
ELSEVIER
DOI: 10.1016/j.brainres.2007.09.080
关键词
rhesus macaque genome array; laser capture microdissection; lateral geniculate nucleus; gene expression; monkey cross-species hybridization
This report is based on an ongoing study to examine gene expression differences in monkey lateral geniculate nucleus (LGN). Here, samples from an Old World species, the vervet monkey (Cercopithecus aethiops), were cross-hybridized to the Rhesus Macaque Genome Array (Affymetrix). Microarray analysis was performed using laser capture microdissected populations of individual neuronal cell bodies isolated from the LGN compared to heterogeneous samples from whole lamina. Our results indicated that cross-species hybridization of microdissected brain tissue samples from vervet monkeys to the Rhesus array produced reliable and biologically relevant data sets. We present the first list of genes enriched in the large neuronal cell bodies of the LGN. We found that these cell bodies are concentrated with genes involved in metabolic processes and protein synthesis, whereas signaling molecules including chemokines and integrins were expressed at higher levels within heterogeneous samples. Our data set also provides support for a contribution of Writ signaling in adult monkey LGN. (C) 2007 Elsevier B.V. All rights reserved.
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