期刊
BRAIN RESEARCH
卷 1185, 期 -, 页码 275-282出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2007.09.009
关键词
estrogen receptor beta; DPN; stroke; neuroprotection; behavior
Selective estrogen receptor (ER) agonists can indicate which receptor subtypes are implicated in neuroprotection. This study investigated the contribution of ER beta, using the selective agonist diarylpropiolnitrile (DPN), in a rat model of stroke. Lister Hooded rats were ovariectomized and implanted with mini-pumps containing either DPN (8 mg kg(-1) day(-1)) (n = 7) or vehicle (n = 5). Sensorimotor function was assessed using a neurological score and the spontaneous forelimb use asymmetry (cylinder) test. One week later the animals received a middle cerebral artery occlusion (MCAO), and T-2-weighted MRI at 48 h post-MCAO quantified ischemic damage. Functional recovery was tested for 7 days post-MCAO and brains processed for histological verification of infarct size. The MRI images revealed no significant differences in hemispheric lesion volumes between vehicle- and DPN-treated groups (35.6 +/- 3.5% and 30.8 +/- 1.7%, respectively [mean +/- SEM]; Student's unpaired t-test df=10, t=-1.357, p = 0.453); this was confirmed histologically at 7 days. MCAO induced significant decline in neurological score performance (from 22 to 11 at 2 h post-MCAO) in the vehicle-treated animals, which was not significantly influenced by DPN. MCAO also induced significant changes in forelimb use in the cylinder test (10% reduction in contralateral, 20% reduction in both, and 30% increase in ipsilateral forelimb use) but this response was not significantly different between groups [F(1,1) =2.929, p = 0.118, repeated-measures ANOVA]. in conclusion, pretreatment with the ER beta agonist DPN did not influence infarct size or sensorimotor function in rats exposed to MCAO. (C) 2007 Elsevier B.V. All rights reserved
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