期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 83A, 期 4, 页码 897-905出版社
WILEY-LISS
DOI: 10.1002/jbm.a.31403
关键词
injectable; ascorbate; transforming growth factor beta-3 (TGF beta-3); chondrocytes; cartilage
This study evaluated the potential of using poly(NiPAAm-co-AAc) as an injectable drug delivery carrier and a cell therapeutic agent in the form of a supporting matrix for the chondrogenic differentiation of rabbit chondrocytes. In particular, rabbit chondrocytes were embedded in hydrogels containing a combination of ascorbate and transforming growth factor beta-3 (TGF beta-3). Hydrogel constructs containing embedded cells either without ascorbate or a combination of ascorbate and TGF beta-3 were used as controls to determine the effects of ascorbate and TGF beta-3 on chondrogenic differentiation. The level of cartilage associated ECM proteins was examined using immunohistochemical staining for collagen type II as well as by Safranin-O and Alcian blue (GAG) staining. The results showed that ascorbate is an important factor for preparing cartilage constructs because of its action on chondrocyte phenotype modulation and proliferation. (c) 2007 Wiley Periodicals, Inc.
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