期刊
BIOSENSORS & BIOELECTRONICS
卷 23, 期 5, 页码 627-632出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2007.07.015
关键词
surface plasmon resonance; biosensor; photoelastic modulator
In this study, a novel phase-sensitive surface plasmon resonance (SPR) setup, based on temporal modulation of a pumping beam by a photoelastic modulator, and subsequent extraction of phase information at the second and the third harmonics of the modulation frequency, has been developed to study biomolecular interactions on SPR-supporting gold. We demonstrated that the design setup provides ultra-high phase sensitivity, together with a wide dynamic range of measurements. In particular, the proposed scheme was used to study real-time interaction of biotin-protein and streptavidin-BSA complexes. We have found that the proposed technique has a detection limit as high as 2.89 x 10(-7) in terms of refractive index units (RI U). In terms of biosensing performance, a detection sensitivity of 1.3 nM from the streptavidin-inaleimide/thiolated BSA complex binding reaction has also been demonstrated. (c) 2007 Elsevier B.V. All rights reserved.
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