期刊
DEVELOPMENT
卷 134, 期 24, 页码 4479-4489出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.008979
关键词
NCS-1; neurite outgrowth; activity-dependent calcium signals; fura-2 imaging; Lymnaea stagnalis
Neurite extension and branching are affected by activity-dependent modulation of intracellular Ca2+, such that an optimal window of [Ca2+](i) is required for outgrowth. Our understanding of the molecular mechanisms regulating this optimal [Ca2+](i) remains unclear. Taking advantage of the large growth cone size of cultured primary neurons from pond snail Lymnaea stagnalis combined with dsRNA knockdown, we show that neuronal calcium sensor-1 (NCS-1) regulates neurite extension and branching, and activity-dependent Ca2+ signals in growth cones. An NCS-1 C-terminal peptide enhances only neurite branching and moderately reduces the Ca2+ signal in growth cones compared with dsRNA knockdown. Our findings suggest that at least two separate structural domains in NCS-1 independently regulate Ca2+ influx and neurite outgrowth, with the C-terminus specifically affecting branching. We describe a model in which NCS-1 regulates cytosolic Ca2+ around the optimal window level to differentially control neurite extension and branching.
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