期刊
BIOCHEMICAL PHARMACOLOGY
卷 74, 期 12, 页码 1738-1746出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2007.08.020
关键词
PPAR alpha; apolipoprotein; HDL; triglyceride; KRP; HepaRG
Peroxisome proliferator-activated receptor alpha (PPAR alpha) is a key regulator in hepatic lipid metabolism and a potential therapeutic target for dyslipidemia. However, in humans hepatic PPAR alpha-regulated genes remain unclear. To investigate the effect of PPAR alpha agonism on mRNA expressions of lipid metabolism-related genes in human livers, a potent PPAR alpha agonist, KRP-101 (KRP), was used to treat the human hepatoma cell line, HepaRG cells. KRP did not affect AOX or L-PBE, which are involved in peroxisomal beta-oxidation. KRP increased L-FABP, CPT1A, VLCAD, and PDK4, which are involved in lipid transport or oxidation. However, the EC50 values (114-2500 nM) were > 10-fold weaker than the EC50 value (10.9 nM) for human PPAR alpha in a transactivation assay. To search for more sensitive genes, we determined the mRNA levels of apolipoproteins, apoA-I, apoA-II, apoA-IV, apoA-V, and apoC-III. KRP had no or little effect on apoA-I, apoC-III, and apoA-II. Interestingly, KRP increased apoA-IV (EC50, 0.99 nM) and apoA-V (EC50, 0.29 nM) with high sensitivity. We identified apoA-IV as a PPA alpha-upregulated gene in a study using PPAR alpha siRNA. Moreover, when administered orally to dogs, KRP decreased the serum triglyceride level and increased the serum apoA-IV level in a dose-dependent manner. These findings suggest that apoA-IV, newly identified as a highly sensitive PPAR alpha-regulated gene in human livers, maybe one of the mechanisms underlying PPAR alpha agonist-induced triglyceride decrease and HDL elevation. (c) 2007 Elsevier Inc. All rights reserved.
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