期刊
TALANTA
卷 74, 期 3, 页码 387-392出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2007.09.023
关键词
amperometric sensor; electropolymerization; carbon nanofiber; thionine; ethanol
资金
- NIGMS NIH HHS [5R44GM62077-03] Funding Source: Medline
Thionine had strong interaction with carbon nanofiber (CNF) and was used in the non-covalent functionalization of carbon nanofiber for the preparation of stable thionine-CNF nanocomposite with good dispersion. With a simple one-step electrochemical polymerization of thionine-CNF nanocomposite and alcohol oxidase (AOD), a stable poly(thionine)-CNF/AOD biocomposite film was formed on electrode surface. Based on the excellent catalytic activity of the biocomposite film toward reduction of dissolved oxygen, a sensitive ethanol biosensor was proposed. The ethanol biosensor could monitor ethanol ranging from 2.0 to 252 mu M with a detection limit of 1.7 mu M. It displayed a rapid response, an expanded linear response range as well as excellent reproducibility and stability. The combination of catalytic activity of CNF and the promising feature of the biocomposite with one-step non-manual technique favored the sensitive determination of ethanol with improved analytical capabilities. (c) 2007 Published by Elsevier B.V.
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