期刊
JOURNAL OF CELL BIOLOGY
卷 179, 期 6, 页码 1095-1103出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200710058
关键词
-
类别
资金
- Intramural NIH HHS Funding Source: Medline
- NIGMS NIH HHS [T32 GM008613, R01-GM53034, R01 GM053034, T32-GM08613] Funding Source: Medline
- NINDS NIH HHS [R01 NS041617, R01-NS41617] Funding Source: Medline
Although bulk chromatin is thought to have limited mobility within the interphase eukaryotic nucleus, directed long-distance chromosome movements are not unknown. Cajal bodies (CBs) are nuclear sub-organelles that nonrandomly associate with small nuclear RNA ( snRNA) and histone gene loci in human cells during interphase. However, the mechanism responsible for this association is uncertain. In this study, we present an experimental system to probe the dynamic interplay of CBs with a U2 snRNA target gene locus during transcriptional activation in living cells. Simultaneous four-dimensional tracking of CBs and U2 genes reveals that target loci are recruited toward relatively stably positioned CBs by long-range chromosomal motion. In the presence of a dominant-negative mutant of beta-actin, the repositioning of activated U2 genes is markedly inhibited. This supports a model in which nuclear actin is required for these rapid, long-range chromosomal movements.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据