期刊
FEBS LETTERS
卷 581, 期 30, 页码 5852-5858出版社
WILEY
DOI: 10.1016/j.febslet.2007.11.061
关键词
thermogenic plants; mitochondria; respiration alternative oxidase; skunk cabbage; pyruvate
The cyanide-resistant alternative oxidase (AOX) is a homodimeric protein whose activity can be regulated by the oxidation/reduction state and by a-keto acids. To further clarify the role of AOX in the skunk cabbage, Symplocarpus renifolius, we have performed expression and functional analyses of the encoding gene. Among the various tissues in the skunk cabbage, SrAOX transcripts were found to be specifically expressed in the thermogenic spadix. Moreover, our data demonstrate that the SrAOX protein exists as a non-covalently associated dimer in the thermogenic spadix, and is more sensitive to pyruvate than to other carboxylic acids. Our results suggest that the pyruvate-mediated modi. cation of SrAOX activity plays a significant role in thermoregulation in the skunk cabbage. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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