期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 364, 期 4, 页码 844-849出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2007.10.091
关键词
Prp19p; adipocytes; lipid droplet; nucleus; localization signal
Prp19p is a protein found in the nucleus, cytosol or lipid droplets depending on the cell type. Prp19p participates in pre-mRNA splicing, in neuronal/astroglial cell fate decisions or in adipocyte lipid droplet biogenesis. In this study, the motifs of Prp19p that are necessary for its localization to lipid droplets or the nucleus in 3T3-L1 adipocytes are investigated using a series of truncated mutants of Prp19p that were fused to EGFP and transiently introduced into differentiated 3T3-L1 adipocytes. Immunofluorescence microscopy revealed that a domain of amino acids 167-250 is necessary for the recruitment of Prp19p to lipid droplets and that a domain of amino acids 1-166 is necessary for the recruitment of Prp19p to a nucleus. (C) 2007 Elsevier Inc. All rights reserved.
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