期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 99, 期 2, 页码 351-367出版社
WILEY
DOI: 10.1002/bit.21567
关键词
cell-free protein synthesis; disulfide bond; protein folding; production platform; fusion protein; patient specific vaccine; E. coli membrane vesicles
The use of cell-free protein synthesis (CFPS) for recombinant protein production is emerging as an important technology. For example, the openness of the cell-free system allows control of the reaction environment to promote folding of disulfide bonded proteins in a rapid and economically feasible format. These advantages make cell-free protein expression systems particularly well suited for producing patient specific therapeutic vaccines or antidotes in response to threats from natural and man-made biological agents and for pharmaceutical proteins that are difficult to produce in living cells. In this work we assess the versatility of modern cell-free methods, optimize expression and folding parameters, and highlight the importance of rationally designed plasmid templates for producing mammalian secreted proteins, fusion proteins, and antibody fragments in our E. coli-based UPS system. Two unique UPS platforms were established by developing standardized extract preparation protocols and generic cell-free reaction conditions. Generic reaction conditions enabled all proteins to express well with the best therapeutic protein yield at 710 mu g/mL, an antibody fragment at 230 mu g/mL, and a vaccine fusion protein at 300 mu g/mL; with the majority correctly folded. Better yields were obtained when cell-free reaction conditions were optimized for each protein. Establishing general UPS platforms enhances the potential for cell-free protein synthesis to reliably produce complex protein products at low production and capital costs with very rapid process development timelines.
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