期刊
JOURNAL OF GENERAL PLANT PATHOLOGY
卷 74, 期 1, 页码 71-75出版社
SPRINGER TOKYO
DOI: 10.1007/s10327-007-0061-5
关键词
Ralstonia solanacearum; type II secretion system; xylem vessels
Ralstonia solanacearum strain OE1-1 (OE1-1) systemically invades tobacco plants and causes bacterial wilt. A type II secretion system (T2SS)-deficient mutant of OE1-1, derived from EZ::TN < KAN-2 > transposon-insertion, retained the ability of the parent strain to produce exopolysaccharide in vitro and grow in intercellular spaces immediately after invasion of host plants, but lost the ability to systemically infect the host. With transmission electron microscopy, the mutant was not observed in xylem vessels. These findings suggest that the T2SS contributes to systemic infection by enabling the bacteria to invade xylem vessels.
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