期刊
AMERICAN JOURNAL OF RHINOLOGY & ALLERGY
卷 26, 期 6, 页码 433-438出版社
SAGE PUBLICATIONS INC
DOI: 10.2500/ajra.2012.26.3814
关键词
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资金
- Ono Cancer Research Fund
- Suhara Memorial Foundation
- Pancreas Research Foundation of Japan
- Ministry of Education, Culture, Sports, Science, and Technology
- Ministry of Health, Labor, and Welfare of Japan
- Japan Science and Technology Agency
- Program for developing the supporting system for upgrading education and research
- Grants-in-Aid for Scientific Research [24659750, 23390398, 23590404, 24791795] Funding Source: KAKEN
Background: Thymic stromal lymphopoietin (TSLP) acts as a master switch for allergic inflammation and plays a key role in allergic diseases, including allergic rhinitis. Double-stranded RNA (dsRNA) recognized by Toll-like receptor 3 (TLR3) strongly activates TSLP release from human nasal epithelial cells (HNECs). Hop (Humulus lupulus L.) extracts have been shown to have potent pharmacologic effects on inflammation. Methods: To investigate whether a hop water extract (HWE) prevents TSLP release from HNECs, human telomerase reverse transcriptase (hTERT)-transfected HNECs, used as a model of normal HNECs, were pretreated with HWE before treatment with the TLR3 ligand Polyinosine-polycytidylic acid (poly[I:C]). Results: In the hTERT-transfected HNECs, treatment with HWE significantly reduced poly(I:C)-induced production and release of TSLP in a dose-dependent manner, as well as dexamethasone. Treatment with the protein kinase C (PKC) inhibitor GF109203X and NF-kappa B inhibitor IMD-0354 also reduced poly(I:C)-induced TSLP release from hTERT-transfected HNECs. Treatment with HWE efficiently prevented up-regulation of PKC activity by 12-O-tetradecanoyl phorbol-13-acetate but not NF-kappa B activity induced by IL-1 beta in hTERT-transfected HNECs. Conclusion: Our results clearly indicated that HWE inhibited dsRNA-induced production and release of TSLP via a PKC signal pathway in HNECs and it may have potent preventive effects against allergic rhinitis.
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