期刊
AMERICAN JOURNAL OF RHINOLOGY & ALLERGY
卷 23, 期 6, 页码 E10-E13出版社
OCEAN SIDE PUBLICATIONS INC
DOI: 10.2500/ajra.2009.23.3389
关键词
Acidic; airway surface liquid (ASL); asthma; mucosa; pH stat; proton secretion; sinusitis; Ussing chamber
资金
- NCCIH NIH HHS [P01 AT002620, P01 AT002620-01] Funding Source: Medline
- NHLBI NIH HHS [R21 HL089196-01A2, R01 HL086323-02, 5T35 HL007809, R21 HL089196, R01 HL086323] Funding Source: Medline
Background: Proton (H+) secretion and the HVCN1 H+ channel are part of the innate host defense mechanism of the airways. The objective of this study was to determine H+ secretion in asthmatic and nonasthmatic patients with chronic rhinosinusitis (CRS) in freshly excised human sinonasal tissue. Methods: Nasal or sinus mucosa from subjects with three different conditions (normal, CRS, and CRS with asthma) was harvested during sinus surgery. The equilibrium pH and the rate of H+ secretion were measured in an Ussing chamber using the pH-stat titration technique. Results: Nasal epithelia isolated from subjects with CRS and asthma had a mucosal equilibrium pH = 6.95 (n = 5), which was significantly lower than in normal subjects (7.35 +/- 0.21; n = 5) or from subjects with CRS without asthma (7.33 +/- 0.15 In = 5). Nasal epithelia from CRS with asthma (n = 5) secreted H+ at a rate of 135 +/- 46 nmol.min(-1).cm(-2). This rate was significantly higher compared with normal (73 +/- 39 nmol.min(-1).cm(-2); n = 8) or CRS without asthma (51 +/- 28 nmol.min(-1).cm(-2); n = 7). Mucosal addition of the HVCN1 blocker ZnCl2 blocked H+ secretion by 70% in normal, 53% in CRS without asthma, and by 51% in CRS with asthma. In contrast, measures in sinus tissues were unaffected by the disease condition. Conclusion: Freshly excised human nasal and sinus epithelia secrete acid. Nasal (but not sinus) tissues from asthmatic CRS patients showed lower mucosal pH values and higher rates of H+ secretion than CRS and normal subjects. The increased acid secretion might contribute to epithelial injury in CRS patients with asthma. (Am J Rhinol Allergy 23, e10-e13, 2009; doi: 10.2500/ajra.2009.23.3389)
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