期刊
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
卷 47, 期 3, 页码 306-314出版社
AMER THORACIC SOC
DOI: 10.1165/rcmb.2011-0297OC
关键词
lung; epithelium; Wnt3a; fibrosis; epithelial to mesenchymal transition
资金
- National Institutes of Health [T32 HL076122, R01 HL085464]
Recent studies suggest the importance of the transition of airway epithelial cells (EMT) in pulmonary fibrosis, and also indicate a role for Wingless protein (Wnt)/beta-catenin signaling in idiopathic pulmonary fibrosis. We investigated the possible role of the Wnt signaling pathway in inducing EMT in lung epithelial cells, and sought to unravel the role of c-Jun-N-terminal-kinase-1 (JNK1). The exposure of C10 lung epithelial cells or primary mouse tracheal epithelial cells (MTECs) to Wnt3a resulted in increases in JNK phosphorylation and nuclear beta-catenin content. Because the role of beta-catenin as a transcriptional coactivator is well established, we investigated T-cell factor/lymphocyte-enhancement factor (TCF/LEF) transcriptional activity in C10 lung epithelial cells after the activation of Wnt. TCF/LEF transcriptional activity was enhanced after the activation of Wnt, and this increase in TCF/LEF transcriptional activity was diminished after the small interfering (si)RNA-mediated ablation of JNK. The activation of the Wnt pathway by Wnt3a, or the expression of either wild-type or constitutively active beta-catenin (S37A), led to the activation of an EMT transcriptome, manifested by the increased mRNA expression of CArG box-binding factor-A, fibroblast-specific protein (FSP)-1, alpha-smooth muscle actin (alpha-SMA), and vimentin, increases in the content of alpha-SMA and FSP1, and the concomitant loss of zona occludens-1. The siRNA-mediated ablation of beta-catenin substantially decreased Wnt3a-induced EMT. The siRNA ablation of JNK1 largely abolished Wnt3a, beta-catenin, and beta-catenin S37a-induced EMT. In MTECs lacking Jnk1, Wnt3a-induced increases in nuclear beta-catenin, EMT transcriptome, and the content of alpha-SMA or FSP1 were substantially diminished. These data show that the activation of the Wnt signaling pathway is capable of inducing an EMT program in lung epithelial cells through beta-catenin, and that this process is controlled by JNK1.
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