期刊
NUCLEIC ACIDS RESEARCH
卷 36, 期 4, 页码 1120-1128出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkm1125
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资金
- NCRR NIH HHS [C06 RR-16572-01, C06 RR016572] Funding Source: Medline
- NIGMS NIH HHS [GM54215, R01 GM054215] Funding Source: Medline
DNA bending and torsional deformations that often occur during its functioning inside the cell can cause local disruptions of the regular helical structure. The disruptions created by negative torsional stress have been studied in detail, but those caused by bending stress have only been analyzed theoretically. By probing the structure of very small DNA circles, we determined that bending stress disrupts the regular helical structure when the radius of DNA curvature is smaller than 3.5 nm. First, we developed an efficient method to obtain covalently closed DNA minicircles. To detect structural disruptions in the minicircles we treated them by single-strand-specific endonucleases. The data showed that the regular DNA structure is disrupted by bending deformation in the 64-65-bp minicircles, but not in the 85-86-bp minicircles. Our results suggest that strong DNA bending initiates kink formation while preserving base pairing.
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