Methylome of Fetal and Maternal Monocytes and Macrophages at the Feto-Maternal Interface

Problem Decidual macrophages (dMf) of the mother and placental macrophages (Hofbauer cells, HC) of the fetus are deployed at a critical location: the feto-maternal interface. This study was conducted to compare the DNA methylome of maternal and fetal monocytes, dMf, and HC and thereby to determine the immunobiological importance of DNA methylation in pregnancy. Method of Study Paired samples were obtained from normal pregnant women at term not in labor and their neonates. Maternal monocytes (MMo) and fetal monocytes (FMo) were isolated from the peripheral blood of mothers and fetal cord blood, respectively. dMf and HC were obtained from the decidua of fetal membranes and placentas, respectively. DNA methylation profiling was performed using the Illumina Infinium Human Methylation27 BeadChip. Quantitative real-time PCR and Western Blot were performed for validation experiments. Results (i) Significant differences in DNA methylation were found in each comparison (MMo versus FMo, 65 loci; dMf versus HC, 266 loci; MMo versus dMf, 199 loci; FMo versus HC, 1030 loci). (ii) Many of the immune response-related genes were hypermethylated in fetal cells (FMo and HC) compared to maternal cells (MMo and dMf). (iii) Genes encoding markers of classical macrophage activation were hypermethylated, and genes encoding alternative macrophage activation were hypomethylated in dMf and HC compared to MMo and FMo, respectively. (iv) mRNA expressions of DNMT1, DNMT3A, and DNMT3B were significantly lower in dMf than in HC. (v) 5-azacytidine treatment increased expression of INCA1 in dMf. Conclusions The findings herein indicate that DNA methylation patterns change during monocytemacrophage differentiation at the feto-maternal interface. It is also suggested that DNA methylation is an important component of the biological machinery conferring an anti-inflammatory phenotype to macrophages at the feto-maternal interface.