4.7 Article

Study of cell behaviour on a cellulose anti-adhesive substratum

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CELLULOSE
卷 15, 期 2, 页码 347-357

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SPRINGER
DOI: 10.1007/s10570-007-9182-4

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carboxymethylcellulose; hydroxypropylmethylcellulose; three-dimensional culture; cell adhesion; cell proliferation; apoptosis

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Carboxymethylcellulose (CMC) is used as an anti-adhesive in several biomaterial applications. We have shown that an original bi-layered hydroxypropylmethylcellulose (HPMC)-CMC-coated substratum (CEL) is suitable for studying the molecular mechanisms underlying cell-substratum and cell-cell interactions. The present work was carried out to assess the anti-adhesive properties of a CEL substratum by analysing the behaviour of three types of adherent cells (Swiss 3T3 fibroblasts, MC-3T3-E1 pre-osteoblasts and B16F10 highly metastatic melanoma cells). Tissue culture polystyrene (tPS) was used as an adhesive control and poly(2-hydroxyethyl methacrylate) PolyHEMA as an anti-adhesive control. Cell morphology, growth, cell cycle analysis and apoptosis were compared. The proliferation of cells on CEL was significantly decreased, cells were arrested in the G1 phase and underwent apoptosis (except for melanoma cells) 48 h post-seeding. We conclude that CEL is an effective anti-adhesive cellulose biomaterial that gives reproducible and demonstratable results, especially for apoptosis. Its advantage as a coating for culture devices is its long shelf-live. It may therefore also be a very good candidate for other biomedical applications requiring adhesion-resistant substrata.

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