期刊
PLANTA
卷 227, 期 5, 页码 1037-1046出版社
SPRINGER
DOI: 10.1007/s00425-007-0678-9
关键词
calcium; development; embryogenesis; Fucus; phospholipase C; reactive oxygen species
资金
- Biotechnology and Biological Sciences Research Council [P18266, REI20579] Funding Source: Medline
- NERC [MBA010001] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [REI20579, P18266] Funding Source: researchfish
- Natural Environment Research Council [MBA010001] Funding Source: researchfish
We report the existence of a tip-high reactive oxygen species (ROS) gradient in growing Fucus serratus zygotes, using both 5-(and 6-) chloromethyl-2',7'-dichlorodihydrofluorescein and nitroblue tetrazolium staining to report ROS generation. Suppression of the ROS gradient inhibits polarized zygotic growth; conversely, exogenous ROS generation can redirect zygotic polarization following inhibition of endogenous ROS. Confocal imaging of fluo-4 dextran distributions suggests that the ROS gradient is interdependent on the tip-high [Ca2+](cyt) gradient which is known to be associated with polarized growth. Our data support a model in which localized production of ROS at the rhizoid tip stimulates formation of a localized tip-high [Ca2+](cyt) gradient. Such modulation of intracellular [Ca2+](cyt) signals by ROS is a common motif in many plant and algal systems and this study extends this mechanism to embryogenesis.
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