期刊
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
卷 302, 期 8, 页码 R990-R995出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00659.2011
关键词
collagen; cross-linking; hydroxylyslpyridinoline; hydroxyproline; stiffness
类别
资金
- Midwestern University Office of Research and Sponsored Programs
- College of Health Sciences
Carroll CC, Whitt JA, Peterson A, Gump BS, Tedeschi J, Broderick TL. Influence of acetaminophen consumption and exercise on Achilles tendon structural properties in male Wistar rats. Am J Physiol Regul Integr Comp Physiol 302: R990-R995, 2012. First published February 22, 2012; doi: 10.1152/ajpregu.00659.2011.-Chronic consumption of acetaminophen (APAP) during exercise training leads to a reduction in tendon stiffness and modulus compared with a placebo. We explored whether this effect could be due to a reduction in tendon collagen content or cross-linking. Ten-week-old male Wistar rats (n = 50) were divided into placebo or APAP groups and into sedentary or treadmill-exercised groups. APAP (200 mg/kg) or saline was administered once daily by oral gavage. Rats in the exercise groups ran on a treadmill 5 days per week for 8 wk with progression to 60 min per day, 20 m/min, and 8 degrees incline. After 8 wk, lyophilized Achilles tendon samples were assayed for the collagen-specific amino acid hydroxyproline and cross-linking [hydroxylyslpyridinoline (HP)] content by high-performance liquid chromatrography. Collagen content was not influenced by exercise or APAP (P > 0.05). Compared with placebo, tendon water content was 7% (P = 0.006, main effect) lower in animals consuming APAP (placebo: 54.79 +/- 0.8%, APAP: 50.89 +/- 1.2%). HP in the Achilles tendon was 36% greater (sedentary: 141 +/- 15, exercise: 204 +/- 26 mmol/mol collagen) in the exercise-trained rats independent of drug treatment (P = 0.020, main effect). Independent of exercise, HP content was 33% lower (P = 0.032, main effect) in the animals consuming APAP (placebo: 195 +/- 21, APAP: 140 +/- 19 mmol/mol collagen). Our data suggests that chronic consumption of APAP results in a reduction in collagen cross-linking and a loss of tissue water independent of chronic exercise. This reduction in cross-linking and water content could contribute to the decrease in tendon stiffness noted in humans chronically consuming APAP.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据