期刊
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
卷 295, 期 2, 页码 R642-R648出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00852.2007
关键词
exercise; heat shock; stress
类别
资金
- NIAMS NIH HHS [AR-40155] Funding Source: Medline
- NIGMS NIH HHS [T32-GM007198, T32 GM007198] Funding Source: Medline
The purpose of the present study was 1) to develop a stable model for measuring contraction-induced elevations in mRNA in single skeletal muscle fibers and 2) to utilize this model to investigate the response of heat shock protein 72 (HSP72) mRNA following an acute bout of fatiguing contractions. Living, intact skeletal muscle fibers were microdissected from lumbrical muscle of Xenopus laevis and either electrically stimulated for 15 min of tetanic contractions ( EX; n = 26) or not stimulated to contract ( REST; n = 14). The relative mean developed tension of EX fibers decreased to 29 +/- 7% of initial peak tension at the stimulation end point. Following treatment, individual fibers were allowed to recover for 1 ( n = 9), 2 ( n = 8), or 4 h ( n = 9) prior to isolation of total cellular mRNA. HSP72, HSP60, and cardiac alpha-actin mRNA content were then assessed in individual fibers using quantitative PCR detection. Relative HSP72 mRNA content was significantly ( P < 0.05) elevated at the 2-h postcontraction time point relative to REST fibers when normalized to either HSP60 ( 18.5 +/- 7.5-fold) or cardiac alpha-actin ( 14.7 +/- 4.3-fold), although not at the 1-or 4-h time points. These data indicate that 1) extraction of RNA followed by relative quantification of mRNA of select genes in isolated single skeletal muscle fibers can be reliably performed, 2) HSP60 and cardiac alpha-actin are suitable endogenous normalizing genes in skeletal muscle following contractions, and 3) a significantly elevated content of HSP72 mRNA is detectable in skeletal muscle 2 h after a single bout of fatiguing contractions, despite minimal temperature changes and without influence from extracellular sources.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据