期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 304, 期 1, 页码 H58-H71出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00476.2012
关键词
voltage-gated Ca2+ channels; Ca2+ regulation; myogenic tone; vascular smooth muscle cells
资金
- Canadian Institute of Health Research
El-Rahman RR, Harraz OF, Brett SE, Anfinogenova Y, Mufti RE, Goldman D, Welsh DG. Identification of L- and T-type Ca2+ channels in rat cerebral arteries: role in myogenic tone development. Am J Physiol Heart Circ Physiol 304: H58-H71, 2013. First published October 26, 2012; doi:10.1152/ajpheart.00476.2012.L-type Ca2+ channels are broadly expressed in arterial smooth muscle cells, and their voltage-dependent properties are important in tone development. Recent studies have noted that these Ca2+ channels are not singularly expressed in vascular tissue and that other subtypes are likely present. In this study, we ascertained which voltage-gated Ca2+ channels are expressed in rat cerebral arterial smooth muscle and determined their contribution to the myogenic response. mRNA analysis revealed that the alpha(1)-subunit of L-type (Ca(v)1.2) and T-type (Ca(v)3.1 and Ca(v)3.2) Ca2+ channels are present in isolated smooth muscle cells. Western blot analysis subsequently confirmed protein expression in whole arteries. With the use of patch clamp electrophysiology, nifedipine-sensitive and -insensitive Ba2+ currents were isolated and each were shown to retain electrical characteristics consistent with L- and T-type Ca2+ channels. The nifedipine-insensitive Ba2+ current was blocked by mibefradil, kurtoxin, and efonidpine, T-type Ca2+ channel inhibitors. Pressure myography revealed that L- type Ca2+ channel inhibition reduced tone at 20 and 80 mmHg, with the greatest effect at high pressure when the vessel is depolarized. In comparison, the effect of T-type Ca2+ channel blockade on myogenic tone was more limited, with their greatest effect at low pressure where vessels are hyperpolarized. Blood flow modeling revealed that the vasomotor responses induced by T-type Ca2+ blockade could alter arterial flow by similar to 20-50%. Overall, our findings indicate that L- and T-type Ca2+ channels are expressed in cerebral arterial smooth muscle and can be electrically isolated from one another. Both conductances contribute to myogenic tone, although their overall contribution is unequal.
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